Publications

PrePrint

Allmann S., M. Wargnies, E. Cahoreau, M. Biran, N. Plazolles, P. Morand, E. Pineda, H. Kulyk-Babier, C. Asencio, O. Villafraz, L. Rivière, E. Tetaud, B. Rotureau, A. Mourier, J.-C. Portais, & F. Bringaud.  “Metabolic contest”, a new way to control carbon source preference. BioRxiv 2020 (https://doi.org/10.1101/800839)

Published

2021

Souza ROO, Damasceno FS, Marsiccobetre S, Biran M, Murata G, Curi R, Bringaud F, Silber AM. Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi. PLoS Pathog. 2021 17, e1009495. PubMed PMID:33819309

Michels PAM, Villafraz O, Pineda E, Alencar MB, Cáceres AJ, Silber AM, Bringaud F. Carbohydrate metabolism in trypanosomatids: New insights revealing novel complexity, diversity and species-unique features. Exp Parasitol. 2021 224, 108102. PubMed PMID:33775649

Schenk R, Bachmaier S, Bringaud F, Boshart M. Efficient flavinylation of glycosomal fumarate reductase by its own ApbE domain in Trypanosoma brucei. FEBS J. 2021 , . PubMed PMID:33755328

Wargnies M, Plazolles N, Schenk R, Villafraz O, Dupuy JW, Biran M, Bachmaier S, Baudouin H, Clayton C, Boshart M, Bringaud F. Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase. J Biol Chem. 296, 100548. PubMed PMID:33741344

Villafraz O, Biran M, Pineda E, Plazolles N, Cahoreau E, Ornitz Oliveira Souza R, Thonnus M, Allmann S, Tetaud E, Rivière L, Silber AM, Barrett MP, Zíková A, Boshart M, Portais JC, Bringaud F. Procyclic trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in the presence of physiological amounts of proline. PLoS Pathog. 2021 17, e1009204. PubMed PMID:33647053

2020

Mochizuki K, Inaoka DK, Mazet M, Shiba T, Fukuda K, Kurasawa H, Millerioux Y, Boshart M, Balogun EO, Harada S, Hirayama K, Bringaud F, Kita K. The ASCT/SCS cycle fuels mitochondrial ATP and acetate production in Trypanosoma brucei. Biochim Biophys Acta Bioenerg. 2020 1861, 148283. PubMed PMID:32763239

2019

Su X, Rak M, Tetaud E, Godard F, Sardin E, Bouhier M, Gombeau K, Caetano-Anollés D, Salin B, Chen H, di Rago JP, Tribouillard-Tanvier D. Deregulating mitochondrial metabolite and ion transport has beneficial effects in yeast and human cellular models for NARP syndrome. Hum Mol Genet. 2019 28, 3792-3804. PubMed PMID:31276579

Courtois P, Nabos P, Nzoumbou-Boko R, Reix CE, Dauchy FA, Daulouede S, Bringaud F, Robinson DR, Vincendeau P. Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns). J Vis Exp. 2019 , . PubMed PMID:31009012

Florini F, Naguleswaran A, Gharib WH, Bringaud F, Roditi I. Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei. Nucleic Acids Res. 2019 47, 1725-1739. PubMed PMID:30544263

Fernandes PM, Kinkead J, McNae IW, Bringaud F, Michels PAM, Walkinshaw MD. The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction. Biochem J. 2019 476, 179-191. PubMed PMID:30404924

2018

Wargnies M, Bertiaux E, Cahoreau E, Ziebart N, Crouzols A, Morand P, Biran M, Allmann S, Hubert J, Villafraz O, Millerioux Y, Plazolles N, Asencio C, Rivière L, Rotureau B, Boshart M, Portais JC, Bringaud F. Gluconeogenesis is essential for trypanosome development in the tsetse fly vector. PLoS Pathog. 2018 14, e1007502. PubMed PMID:30557412

Pineda E, Thonnus M, Mazet M, Mourier A, Cahoreau E, Kulyk H, Dupuy JW, Biran M, Masante C, Allmann S, Rivière L, Rotureau B, Portais JC, Bringaud F. Glycerol supports growth of the Trypanosoma brucei bloodstream forms in the absence of glucose: Analysis of metabolic adaptations on glycerol-rich conditions. PLoS Pathog. 2018 14, e1007412. PubMed PMID:30383867

Millerioux Y, Mazet M, Bouyssou G, Allmann S, Kiema TR, Bertiaux E, Fouillen L, Thapa C, Biran M, Plazolles N, Dittrich-Domergue F, Crouzols A, Wierenga RK, Rotureau B, Moreau P, Bringaud F. De novo biosynthesis of sterols and fatty acids in the Trypanosoma brucei procyclic form: Carbon source preferences and metabolic flux redistributions. PLoS Pathog. 2018 14, e1007116. PubMed PMID:29813135

Marchese L, Nascimento JF, Damasceno FS, Bringaud F, Michels PAM, Silber AM. The Uptake and Metabolism of Amino Acids, and Their Unique Role in the Biology of Pathogenic Trypanosomatids. Pathogens. 2018 7, . PubMed PMID:29614775

Kovářová J, Pountain AW, Wildridge D, Weidt S, Bringaud F, Burchmore RJS, Achcar F, Barrett MP. Deletion of transketolase triggers a stringent metabolic response in promastigotes and loss of virulence in amastigotes of Leishmania mexicana. PLoS Pathog. 2018 14, e1006953. PubMed PMID:29554142

2017

Garcia-Silva MR, Sollelis L, MacPherson CR, Stanojcic S, Kuk N, Crobu L, Bringaud F, Bastien P, Pagès M, Scherf A, Sterkers Y. Identification of the centromeres of Leishmania major: revealing the hidden pieces. EMBO Rep. 2017 18, 1968-1977. PubMed PMID:28935715

Smith TK, Bringaud F, Nolan DP, Figueiredo LM. Metabolic reprogramming during the Trypanosoma brucei life cycle. F1000Res. 2017 6, . PubMed PMID:28620452

Thonnus M, Guérin A, Rivière L. A multigene family encoding surface glycoproteins in Trypanosoma congolense. Microb Cell. 2017 4, 90-97. PubMed PMID:28357394

de Taffin de Tilques M, Tribouillard-Tanvier D, Tétaud E, Testet E, di Rago JP, Lasserre JP. Overexpression of mitochondrial oxodicarboxylate carrier (ODC1) preserves oxidative phosphorylation in a yeast model of Barth syndrome. Dis Model Mech. 2017 10, 439-450. PubMed PMID:28188263

Allmann S, Bringaud F. Glycosomes: A comprehensive view of their metabolic roles in T. brucei. Int J Biochem Cell Biol. 2017 85, 85-90. PubMed PMID:28179189

Mantilla BS, Marchese L, Casas-Sánchez A, Dyer NA, Ejeh N, Biran M, Bringaud F, Lehane MJ, Acosta-Serrano A, Silber AM. Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector. PLoS Pathog. 2017 13, e1006158. PubMed PMID:28114403

Harijan RK, Kiema TR, Syed SM, Qadir I, Mazet M, Bringaud F, Michels PAM, Wierenga RK. Crystallographic substrate binding studies of Leishmania mexicana SCP2-thiolase (type-2): unique features of oxyanion hole-1. Protein Eng Des Sel. 2017 30, 225-233. PubMed PMID:28062645

Surve SV, Jensen BC, Heestand M, Mazet M, Smith TK, Bringaud F, Parsons M, Schnaufer A. NADH dehydrogenase of Trypanosoma brucei is important for efficient acetate production in bloodstream forms. Mol Biochem Parasitol. 2017 211, 57-61. PubMed PMID:27717801

2016

Morales J, Hashimoto M, Williams TA, Hirawake-Mogi H, Makiuchi T, Tsubouchi A, Kaga N, Taka H, Fujimura T, Koike M, Mita T, Bringaud F, Concepción JL, Hashimoto T, Embley TM, Nara T. Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids. Proc Biol Sci. 2016 283, . PubMed PMID:27170716

Harijan RK, Mazet M, Kiema TR, Bouyssou G, Alexson SE, Bergmann U, Moreau P, Michels PA, Bringaud F, Wierenga RK. The SCP2-thiolase-like protein (SLP) of Trypanosoma brucei is an enzyme involved in lipid metabolism. Proteins. 2016 84, 1075-96. PubMed PMID:27093562

Štáfková J, Mach J, Biran M, Verner Z, Bringaud F, Tachezy J. Mitochondrial pyruvate carrier in Trypanosoma brucei. Mol Microbiol. 2016 100, 442-56. PubMed PMID:26748989

Jackson AP, Otto TD, Aslett M, Armstrong SD, Bringaud F, Schlacht A, Hartley C, Sanders M, Wastling JM, Dacks JB, Acosta-Serrano A, Field MC, Ginger ML, Berriman M. Kinetoplastid Phylogenomics Reveals the Evolutionary Innovations Associated with the Origins of Parasitism. Curr Biol. 2016 26, 161-172. PubMed PMID:26725202

2015

Creek DJ, Mazet M, Achcar F, Anderson J, Kim DH, Kamour R, Morand P, Millerioux Y, Biran M, Kerkhoven EJ, Chokkathukalam A, Weidt SK, Burgess KE, Breitling R, Watson DG, Bringaud F, Barrett MP. Probing the metabolic network in bloodstream-form Trypanosoma brucei using untargeted metabolomics with stable isotope labelled glucose. PLoS Pathog. 2015 11, e1004689. PubMed PMID:25775470

Bringaud F, Biran M, Millerioux Y, Wargnies M, Allmann S, Mazet M. Combining reverse genetics and nuclear magnetic resonance-based metabolomics unravels trypanosome-specific metabolic pathways. Mol Microbiol. 2015 96, 917-26. PubMed PMID:25753950

Bringaud F, Rogers M, Ghedin E. Identification and analysis of ingi-related retroposons in the trypanosomatid genomes. Methods Mol Biol. 2015 1201, 109-22. PubMed PMID:25388110

Shameer S, Logan-Klumpler FJ, Vinson F, Cottret L, Merlet B, Achcar F, Boshart M, Berriman M, Breitling R, Bringaud F, Bütikofer P, Cattanach AM, Bannerman-Chukualim B, Creek DJ, Crouch K, de Koning HP, Denise H, Ebikeme C, Fairlamb AH, Ferguson MA, Ginger ML, Hertz-Fowler C, Kerkhoven EJ, Mäser P, Michels PA, Nayak A, Nes DW, Nolan DP, Olsen C, Silva-Franco F, Smith TK, Taylor MC, Tielens AG, Urbaniak MD, van Hellemond JJ, Vincent IM, Wilkinson SR, Wyllie S, Opperdoes FR, Barrett MP, Jourdan F. TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei. Nucleic Acids Res. 2015 43, D637-44. PubMed PMID:25300491

2014

Allmann S, Mazet M, Ziebart N, Bouyssou G, Fouillen L, Dupuy JW, Bonneu M, Moreau P, Bringaud F, Boshart M. Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei. PLoS One. 2014 9, e114628. PubMed PMID:25493940

Deramchia K, Morand P, Biran M, Millerioux Y, Mazet M, Wargnies M, Franconi JM, Bringaud F. Contribution of pyruvate phosphate dikinase in the maintenance of the glycosomal ATP/ADP balance in the Trypanosoma brucei procyclic form. J Biol Chem. 2014 289, 17365-78. PubMed PMID:24794874

Porcel BM, Denoeud F, Opperdoes F, Noel B, Madoui MA, Hammarton TC, Field MC, Da Silva C, Couloux A, Poulain J, Katinka M, Jabbari K, Aury JM, Campbell DA, Cintron R, Dickens NJ, Docampo R, Sturm NR, Koumandou VL, Fabre S, Flegontov P, Lukeš J, Michaeli S, Mottram JC, Szöőr B, Zilberstein D, Bringaud F, Wincker P, Dollet M. The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. PLoS Genet. 2014 10, e1004007. PubMed PMID:24516393

2013

Mazet M, Morand P, Biran M, Bouyssou G, Courtois P, Daulouède S, Millerioux Y, Franconi JM, Vincendeau P, Moreau P, Bringaud F. Revisiting the central metabolism of the bloodstream forms of Trypanosoma brucei: production of acetate in the mitochondrion is essential for parasite viability. PLoS Negl Trop Dis. 2013 7, e2587. PubMed PMID:24367711

Škodová I, Verner Z, Bringaud F, Fabian P, Lukeš J, Horváth A. Characterization of two mitochondrial flavin adenine dinucleotide-dependent glycerol-3-phosphate dehydrogenases in Trypanosoma brucei. Eukaryot Cell. 2013 12, 1664-73. PubMed PMID:24142106

Millerioux Y, Ebikeme C, Biran M, Morand P, Bouyssou G, Vincent IM, Mazet M, Riviere L, Franconi JM, Burchmore RJ, Moreau P, Barrett MP, Bringaud F. The threonine degradation pathway of the Trypanosoma brucei procyclic form: the main carbon source for lipid biosynthesis is under metabolic control. Mol Microbiol. 2013 90, 114-29. PubMed PMID:23899193

Capewell P, Monk S, Ivens A, Macgregor P, Fenn K, Walrad P, Bringaud F, Smith TK, Matthews KR. Regulation of Trypanosoma brucei Total and Polysomal mRNA during Development within Its Mammalian Host. PLoS One. 2013 8, e67069. PubMed PMID:23840587

Allmann S, Morand P, Ebikeme C, Gales L, Biran M, Hubert J, Brennand A, Mazet M, Franconi JM, Michels PA, Portais JC, Boshart M, Bringaud F. Cytosolic NADPH homeostasis in glucose-starved procyclic Trypanosoma brucei relies on malic enzyme and the pentose phosphate pathway fed by gluconeogenic flux. J Biol Chem. 2013 288, 18494-505. PubMed PMID:23665470

2012

Ghozlane A, Bringaud F, Soueidan H, Dutour I, Jourdan F, Thébault P. Flux Analysis of the Trypanosoma brucei Glycolysis Based on a Multiobjective-Criteria Bioinformatic Approach. Adv Bioinformatics. 2012 2012, 159423. PubMed PMID:23097667

Peña-Diaz P, Pelosi L, Ebikeme C, Colasante C, Gao F, Bringaud F, Voncken F. Functional characterization of TbMCP5, a conserved and essential ADP/ATP carrier present in the mitochondrion of the human pathogen Trypanosoma brucei. J Biol Chem. 2012 287, 41861-74. PubMed PMID:23074217

Godel C, Kumar S, Koutsovoulos G, Ludin P, Nilsson D, Comandatore F, Wrobel N, Thompson M, Schmid CD, Goto S, Bringaud F, Wolstenholme A, Bandi C, Epe C, Kaminsky R, Blaxter M, Mäser P. The genome of the heartworm, Dirofilaria immitis, reveals drug and vaccine targets. FASEB J. 2012 26, 4650-61. PubMed PMID:22889830

Cornillot E, Hadj-Kaddour K, Dassouli A, Noel B, Ranwez V, Vacherie B, Augagneur Y, Brès V, Duclos A, Randazzo S, Carcy B, Debierre-Grockiego F, Delbecq S, Moubri-Ménage K, Shams-Eldin H, Usmani-Brown S, Bringaud F, Wincker P, Vivarès CP, Schwarz RT, Schetters TP, Krause PJ, Gorenflot A, Berry V, Barbe V, Ben Mamoun C. Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microti. Nucleic Acids Res. 2012 40, 9102-14. PubMed PMID:22833609

Cahoreau E, Peyriga L, Hubert J, Bringaud F, Massou S, Portais JC. Isotopic profiling of ¹³C-labeled biological samples by two-dimensional heteronuclear J-resolved nuclear magnetic resonance spectroscopy. Anal Biochem. 2012 427, 158-63. PubMed PMID:22658961

Millerioux Y, Morand P, Biran M, Mazet M, Moreau P, Wargnies M, Ebikeme C, Deramchia K, Gales L, Portais JC, Boshart M, Franconi JM, Bringaud F. ATP synthesis-coupled and -uncoupled acetate production from acetyl-CoA by mitochondrial acetate:succinate CoA-transferase and acetyl-CoA thioesterase in Trypanosoma. J Biol Chem. 2012 287, 17186-17197. PubMed PMID:22474284

Bringaud F, Barrett MP, Zilberstein D. Multiple roles of proline transport and metabolism in trypanosomatids. Front Biosci (Landmark Ed). 2012 17, 349-74. PubMed PMID:22201748

Gualdrón-López M, Brennand A, Hannaert V, Quiñones W, Cáceres AJ, Bringaud F, Concepción JL, Michels PA. When, how and why glycolysis became compartmentalised in the Kinetoplastea. A new look at an ancient organelle. Int J Parasitol. 2012 42, 1-20. PubMed PMID:22142562

2011

Mazet M, Harijan RK, Kiema TR, Haapalainen AM, Morand P, Morales J, Bringaud F, Wierenga RK, Michels PA. The characterization and evolutionary relationships of a trypanosomal thiolase. Int J Parasitol. 2011 41, 1273-83. PubMed PMID:21907205

Bringaud F, Berriman M, Hertz-Fowler C. TSIDER1, a short and non-autonomous Salivarian trypanosome-specific retroposon related to the ingi6 subclade. Mol Biochem Parasitol. 2011 179, 30-6. PubMed PMID:21664383

Stoffel SA, Alibu VP, Hubert J, Ebikeme C, Portais JC, Bringaud F, Schweingruber ME, Barrett MP. Transketolase in Trypanosoma brucei. Mol Biochem Parasitol. 2011 179, 1-7. PubMed PMID:21570429

2010

Ebikeme C, Hubert J, Biran M, Gouspillou G, Morand P, Plazolles N, Guegan F, Diolez P, Franconi JM, Portais JC, Bringaud F. Ablation of succinate production from glucose metabolism in the procyclic trypanosomes induces metabolic switches to the glycerol 3-phosphate/dihydroxyacetone phosphate shuttle and to proline metabolism. J Biol Chem. 2010 285, 32312-24. PubMed PMID:20702405

Bringaud F, Ebikeme C, Boshart M. Acetate and succinate production in amoebae, helminths, diplomonads, trichomonads and trypanosomatids: common and diverse metabolic strategies used by parasitic lower eukaryotes. Parasitology. 2010 137, 1315-31. PubMed PMID:20028611

2009

Spitznagel D, Ebikeme C, Biran M, Nic a' Bháird N, Bringaud F, Henehan GT, Nolan DP. Alanine aminotransferase of Trypanosoma brucei--a key role in proline metabolism in procyclic life forms. FEBS J. 2009 276, 7187-99. PubMed PMID:19895576

Bringaud F, Berriman M, Hertz-Fowler C. Trypanosomatid genomes contain several subfamilies of ingi-related retroposons. Eukaryot Cell. 2009 8, 1532-42. PubMed PMID:19666780

Rivière L, Moreau P, Allmann S, Hahn M, Biran M, Plazolles N, Franconi JM, Boshart M, Bringaud F. Acetate produced in the mitochondrion is the essential precursor for lipid biosynthesis in procyclic trypanosomes. Proc Natl Acad Sci U S A. 2009 106, 12694-9. PubMed PMID:19625628

Smith M, Bringaud F, Papadopoulou B. Organization and evolution of two SIDER retroposon subfamilies and their impact on the Leishmania genome. BMC Genomics. 2009 10, 240. PubMed PMID:19463167

Oyola SO, Bringaud F, Melville SE. A kinetoplastid BRCA2 interacts with DNA replication protein CDC45. Int J Parasitol. 2009 39, 59-69. PubMed PMID:18723021

2008

Bringaud F, Ghedin E, El-Sayed NM, Papadopoulou B. Role of transposable elements in trypanosomatids. Microbes Infect. 2008 10, 575-81. PubMed PMID:18467144

Coustou V, Biran M, Breton M, Guegan F, Rivière L, Plazolles N, Nolan D, Barrett MP, Franconi JM, Bringaud F. Glucose-induced remodeling of intermediary and energy metabolism in procyclic Trypanosoma brucei. J Biol Chem. 2008 283, 16342-54. PubMed PMID:18430732

Ebikeme CE, Peacock L, Coustou V, Riviere L, Bringaud F, Gibson WC, Barrett MP. N-acetyl D-glucosamine stimulates growth in procyclic forms of Trypanosoma brucei by inducing a metabolic shift. Parasitology. 2008 135, 585-94. PubMed PMID:18371239

2007

Bringaud F, Müller M, Cerqueira GC, Smith M, Rochette A, El-Sayed NM, Papadopoulou B, Ghedin E. Members of a large retroposon family are determinants of post-transcriptional gene expression in Leishmania. PLoS Pathog. 2007 3, 1291-307. PubMed PMID:17907803

Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M. Comparative genomic analysis of three Leishmania species that cause diverse human disease. Nat Genet. 2007 39, 839-47. PubMed PMID:17572675

Abdelbaqi K, Ménard A, Prouzet-Mauleon V, Bringaud F, Lehours P, Mégraud F. Nucleotide sequence of the gyrA gene of Arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates. FEMS Immunol Med Microbiol. 2007 49, 337-45. PubMed PMID:17378897